To verify the efficacy of moist heat sterilization procedures used for laboratory glassware and media, which control is employed? _______________?
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Answer: A. using Bacillus stearo thermo philus
Explanation
Geobacillus stearothermophilus spores are extremely heat-resistant and are used to validate moist heat sterilization. These specialized bacterial spores survive harsh conditions that kill ordinary microbial cells. Failure of spores to germinate after autoclaving proves the cycle achieved effective sterilization. Moist heat sterilization relies on pressurized steam autoclaves operating at high temperatures. Biological indicators provide the highest level of assurance that sterilization parameters were met. Geobacillus stearothermophilus spores serve as the gold standard biological challenge for autoclaves.
Quality assurance protocols require routine biological indicator testing for surgical instrument autoclaves. Ensuring complete spore destruction prevents accidental nosocomial transmission during medical procedures. Documentation of biological controls is mandatory for hospital accreditation and safety compliance. Clostridium tetani is an anaerobic pathogen but is not used as a standard autoclave indicator. Chemical indicators change color with heat or steam exposure but do not test actual spore killing. Methylene blue is a redox dye used in anaerobic jars rather than a sterilization control.
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