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Question no. 5477

Successful integration and expression of a transgene are tracked using a ___________?

Select your answer for: Successful integration and expression of a transgene are tracked using a ___________?
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Answer: D. Reporter

Explanation

Reporter genes produce observable traits or detectable proteins to track the successful integration and expression of transgenes. Scientists link these marker proteins to their genes of interest to verify successful genetic transformation. The distinct phenotype conferred by the reporter confirms that the host cell incorporated foreign DNA. Common reporter genes include green fluorescent protein and the enzyme beta galactosidase. These markers provide a visual or biochemical readout that is easy to quantify in laboratory assays. Molecular biologists insert reporters downstream of target promoters to test regulatory element activity.

Reporter genes are indispensable tools in genetic engineering and pharmaceutical research laboratories. They allow scientists to monitor gene expression dynamics in real time within living model organisms. Industrial biotechnology uses these markers to optimize recombinant protein production yields. A transgene is simply the transferred foreign gene itself rather than the tracking tool. A promoter is a regulatory DNA sequence that initiates transcription rather than signaling success. An enhancer boosts transcription rates rather than serving as a visible detection marker.

Subject
Biology
Category
Biology
Topic
—
Difficulty
medium

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