ELISA represents _________?
Show answer Hide answer
Answer: D. Addition of substrate that is converted into a coloured end product
Explanation
The ELISA technique relies fundamentally on an enzymatic reaction where an added substrate is converted into a colored product. This visible color change provides a quantitative or qualitative measure of the target antigen or antibody present. Enzymes like horseradish peroxidase are commonly conjugated to antibodies to catalyze this specific chromogenic reaction. ELISA stands for enzyme-linked immunosorbent assay, a widely used analytical biochemistry test. Antibodies bind specifically to target molecules, allowing detection of hormones, proteins, or infectious pathogens. Spectrophotometers measure the optical density of the resulting colored solution to determine concentration levels.
ELISA is a standard diagnostic tool used in medical laboratories for screening blood donations and infectious diseases. The method offers high sensitivity and specificity for detecting trace amounts of biological molecules in samples. Researchers across diverse scientific fields rely on immunoassays for quantifying proteins in experimental studies. Radiolabelled antibodies are utilized in radioimmunoassays rather than standard enzyme-linked colorimetric assays. Red blood cells are utilized in hemagglutination assays rather than standard enzyme substrate conversion methods. Complement-mediated cell lysis is characteristic of cytotoxicity tests rather than standard diagnostic ELISA procedures.
Related questions
Q1. What terminology describes the pathological process of liver inflammation___________?
Show answer Hide answer
Answer: B. Necrosis
Necrosis is a form of cell injury that results in the premature death of cells in living tissue by autolysis.
Q2. The hummingbird serves as an example of________________?
Show answer Hide answer
Answer: D. Heterotherm
Hummingbirds exhibit heterothermy by dramatically reducing body temperature during nightly periods of torpor. This metabolic adaptation allows them to conserve vital energy when foraging is impossible. Therefore, they ar...
Q3. Multiple populations representing distinct species inhabiting and interacting within a shared habitat constitute a
Show answer Hide answer
Answer: B. Community
Multiple populations representing distinct species living together and interacting within a shared geographical area form a biological community. Ecological communities emphasize the complex biological interactions, such...
Q4. What constitute the prominent biological concerns of the modern era________?
Show answer Hide answer
Answer: E. All of these
Modern biological concerns encompass a wide array of ecological and human health challenges. Rapid human population growth strains natural resource availability across global ecosystems. Infectious diseases, pollution, a...
Q5. All of the following medical conditions stem from viral infections EXCEPT: ____________?
Show answer Hide answer
Answer: D. Diphtheria
Diphtheria is a severe bacterial infection caused by the bacterium Corynebacterium diphtheriae. Bacterial infections require specific antibiotic treatments rather than antiviral medications. The other listed medical cond...
Q6. What embryonic structures do the yellow cytoplasm regions in ascidian eggs give rise to______________?
Show answer Hide answer
Answer: B. Muscle cells
Ascidian eggs contain localized regions of pigmented cytoplasm that determine cell fates. During early embryonic cleavage, the yellow cytoplasm segregates specifically into destined blastomeres. These specific embryonic...